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PRACTICAL    BIOLOGY    LEARNING    PROGRAM


FOR  SECONDARY   STUDENTS


FORM   FOUR     AND   SIX     2015


program  hii   imetengenezwa  maalumu  kwa  wanafunzi   wa  kidato  cha  nne  na sita,
program   zimeandaliwa  zikiwa  na  maelekezo kamili  katika  practical  husika, hivyo  mwanafunzi  atasoma  maelekezo  hayo  na  kufanya  practical.


ripoti  ya  mwanafunzi  lazima  ionyeshe  matokeo  halisi  ya  jaribio  alilofanya.  pia  azingatie  matumizi  sahihi  ya  tenses   katika  kuandika   taarifa  hiyo….. mwanafunzi  ataandika   solution  alizotumia,  jaribio  analofanya  na  mengineyo,   na  ripoti  ya  mwanafunzi itaweza  kuandikwa  kama  ifuatavyo:

kujua  zaidi  namna  ya  kuandika  ripoti  onana  na  mwalimu   wako  wa  darasa

BONYEZA  LINK  ZIFUATAZO  KUPAKUA    WORK  SHEET  KISHA  FUATA  MAELEKEZO.      JIFUNZE  NA   ELEWA  KWA  UNDANI


ALL  FILES  TYPE………WORD DOCUMENT.

Food tests

Reagent for food tests and enzymes

Reagent for food tests and enzymes

1 Test for starch

Using iodine solution
1. Test for starch

2 Test for glucose

Using Benedict’s reagent
2. Test for glucose

3 Test for protein

The biuret reaction
3. Test for protein

4 Test for lipid

The emulsion test
4. Test for fats

5 How sensitive is the starch test?

A serial dilution of starch solution is tested with iodine

6 Testing food for starch

Samples of different foods are tested with iodine solution

7 A comparison of vitamin C content

The volumes of fruit juices needed to decolourise DCPIP are measured

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Enzymes

Reagent for food tests and enzymes

Reagent for food tests and enzymes

1 Effect of amylase on starch

Disappearance of blue colour from starch solution plus iodine

2 Effect of temperature

Timing the disappearance of the blue colour at different temperatures

3 Effect of pH

Timing the disappearance of blue colour at different pH values

4 Catalase

Liver and yeast are used to decompose hydrogen peroxide

5 Effect of enzyme concentration

Increasing strengths of urease are used to produce ammonia from urea

6 Enzymes in maize fruits

Maize fruits are sectioned and placed on starch-agar. Iodine solution reveals clear areas of starch-agar under the fruit

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Respiration

1 Oxygen uptake

The inability of air from germinating seeds to support combustion

2 Carbon dioxide output

The gas from germinating seeds turns limewater milky

3 Exhaled air (1)

The last fraction of exhaled air will not support combustion

4 Exhaled air (2)

Atmospheric air and exhaled air are bubbled simultaneously through limewater

5 Respiration in living organisms

A manometer reveals uptake of oxygen by germinating seeds

6 Anaerobic respiration

The gas produced by a yeast suspension from which air is excluded turns limewater milky

7 Energy release during respiration

Germinating wheat is packed into vacuum flasks to see if there is any rise in temperature over a few days

8 Changes in mass during germination

Wheat is germinated for about two weeks. Samples are taken at 2-day intervals, dried and weighed

9 Measuring the uptake of oxygen

The respirometer is used to measure the uptake of oxygen in living organisms

10 Temperature effect on respiration

The oxygen uptake of living organisms at different temperatures is measured with the respirometer

11 Oxygen uptake in blowfly larvae

The oxygen uptake of blowfly larvae is measured over a 20 minute period. The air in the respirometer is then renewed and the rate of oxygen uptake compared

12 The effect of temperature on fermentation rate

The respirometer is used to measure the rate of carbon dioxide production from a suspension of yeast in glucose solution at different temperatures

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Photosynthesis

Introduction to experiments on Photosynthesis
1 Production of gas by pondweed

Bubbles escape from the cut stems of Canadian pondweed when illuminated

2 Testing a leaf for starch

The technique of killing and decolourising a leaf and testing it with iodine solution

3 The need for light

Light is excluded from part of a leaf which is tested for starch after a period of illumination

4 The need for chlorophyll

A variegated leaf is tested for starch after a period of illumination

5 The need for carbon dioxide

A potted plant is enclosed in a plastic bag from which carbon dioxide has been absorbed.one of its leaves is tested for starch after a period of illumination

6 Collecting the gas from pondweed

The cut end of a Canadian pondweed shoot is placed in a water-filled test tube. The gas is collected and tested for oxygen

7 Gaseous exchange in leaves

Leaves are enclosed in test-tubes containing hydrogencarbonate indicator. The tubes are illuminated or darkened

8 Gaseous exchange in pond-weed

Leaves are enclosed in test-tubes containing hydrogencarbonate indicator. The tubes are illuminated or darkened

9 The need for mineral elements

Wheat seedlings are grown for two weeks in water cultures lacking essential elements

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Diffusion

1 Diffusion in gases

Diffusion of ammonia is observed in a glass tube using litmus paper

2 Diffusion in liquid

Diffusion of methylene blue through gelatine is observed

3 Diffusion and size

Different size blocks of gelatine mixed with cresol red are immersed in acid to see the progress of the acid through the gelatine

4 Diffusion through a membrane

A dialysis tube filled with starch solution is immersed in iodine solution

5 Control of diffusion

Beetroot discs are heated at increasing temperatures to illustrate the control on diffusion by the cell membrane

6 Two-way diffusion

Ammonia diffuses into gelatine coloured with cresol red which diffuses in the opposite direction

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Osmosis

1 Osmosis

An osmometer made from dialysis tubing and a capillary tube shows osmosis happening

2 Selective permeability

Dialysis tubing containing starch and glucose solutions is immersed in water

3 Turgor

A length of dialysis tube is partly filled with a syrup solution and immersed in water

4 Turgor in plant tissue

Strips of dandelion stalk are immersed in salt solutions of differing strength and changes in curvature observed

5 Turgor in potato tissue

Cylinders of potato tissue are immersed in solutions of differing strength and changes in length are measured

6 Root pressure

Glass tubes are fitted to the cut branches of a potted plant. The levels of liquid in each tube are observed

7 Stomatal movements

Leaf epidermis is irrigated with salt solution to see its effect on guard cells

8 Plasmolysis

Red epidermis from rhubarb petiole is irrigated with sucrose solution and observed under the microscope

9 Surface area and osmosis

Potato cubes with equal volume but different surface area are immersed in water and weighed

Human senses

1 Reaction time

The distance a vertical ruler falls before being gripped is converted to a time interval

2a The blind spot (1)

A dot seems to disappear when its image falls on the blind spot

2b The blind spot (2)

A gap in a line is ‘filled in’ when its image falls on the blind spot

3 Inversion of the image

When a pin is viewed via a pinhole in front of the pin, its image appears to be inverted

4a The iris diaphragm (1)

The iris is observed to reduce the size of the pupil when the eye is exposed to light

4b The iris diaphragm (2)

(Broca’s pupillometer) A pattern of pinholes appears to change when one eye is exposed to light

5 Retinal capillaries

By moving a pinhole about in front of the eye, an image of retinal capillaries appears

6a Binocular vision: eye dominance

A pencil lined up with a window frame appears to ‘jump’ when the dominant eye is closed

6b Binocular vision: double vision

Slight pressure on one eyeball causes a single object to appear as a double image

7 Judgement of distance

The space sequence of coloured pinheads is judged using either one or both eyes

8 Eye and hand co-ordination

A star pattern is traced while looking in a mirror

9 Perception

Two shapes are observed, and demonstrate that the brain makes an interpretation of the image

10 Sensitivity of the skin to touch

Different areas of skin are tested with light touch to see if there are differences in reponse

11 Recognition of separate stimuli

(Spatial discrimination) Different areas of skin are tested with a ‘hairpin’ to see if they can discriminate a double touch from a single touch

12 Sensitivity to temperature

One finger is placed in hot water and another in cold water. Both are then placed in warm water and the sensations compared

13 Location of stimuli

A marble is rolled between crossed fingers to give the sensation of two marbles

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Transport in plants

1 Uptake and evaporation in leaves

The uptake of water by single leaves is measured after coating either, neither or both surfaces with Vaseline

2 Uptake of water by shoots

The uptake of water by a shoot is measured, using a potometer

3 Rates of transpiration

The potometer is used in different conditions to compare rates of uptake by the shoot

4 Rate of transpiration and water uptake

By weighing the shoot and potometer, the uptake and loss of water are compared

5 Uptake of water by an uprooted plant

The potometer is modified to accept a whole plant rather than a cut shoot

6 Conditions affecting evaporation

A simple atmometer is used to investigate the effects of different atmospheric conditions on the rate of evaporation

7 Water tension in the stem

The lower end of the potometer is placed in mercury, which is pulled up the capillary by the transpiration force

8 Pathways for gases in a leaf

A leaf is immersed in hot water to expand and force out any air inside it

9 Evaporation from the leaf surface

Evaporation from the upper and lower leaf surface is compared and correlated with the distribution of stomata

10 To collect and identify the product of transpiration

The shoot of a plant is enclosed in a plastic bag. The liquid which condenses is identified

11 To trace the path of water through a shoot

Shoots are placed in a dye in order to investigate the route it takes through the stem and leaves

12 Conducting pathways through the shoot

A syringe is used to force air through a shoot held under water, Air bubbles show the continuity of the vessels

13 Measuring the transpiration rate of a potted plant

Two potted plants, one in sunlight and one in shadow are weighed at intervals

14 Measuring the transpiration rate of an uprooted plant

Two flasks of water are weighed at intervals. One of them contains a plant

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Germination and tropisms

Introduction
1 The need for oxygen

Cress seeds are sown on moist cotton wool in 2 flasks one of which contains pyrogallic acid and sodium hydroxide

2 Effect of temperature

Maize fruits are germinated in moist blotting paper at different temperatures

3 The need for water

Seeds are left in moist, dry and waterlogged conditions for a week

4 The role of cotyledons

Runner bean embryos attached to varying amounts of cotyledon are germinated on moist blotting paper in jars

5 Use of food reserves in germination

Coleoptiles and endosperm of cereal seedlings and grains are tested for starch and sugar

6 Geotropism in radicles

Pea seedlings are pinned to a clinostat, or a stationary base, with their radicles horizontal

7 The region of growth and response in radicles

Radicles are marked with equidistant lines and left horizontally or vertically for two days

8 Region of detection and response to one-sided gravity in radicles

Different lengths of root tip are excised to see if the radicles still grow and respond to gravity

9 The effect of one-sided lighting on shoots

Hypocotyls of sunflower seedlings are marked and illuminated from one side

10 The effect of one-sided lighting on cress seedlings

Cress seedlings, some of which are decapitated, are illuminated from the side or from above

11 The region of detection and response to one-sided lighting in coleoptiles

Coleoptiles of wheat seedlings, some covered by foil caps, some decapitated, are illuminated from the side

12 Effect of indoleacetic acid on coleoptiles

Indoleacetic acid in lanolin is applied to intact coleoptiles. A control is conducted with plain lanolin

13 The effect of indoleacetic acid on wheat coleoptiles

The tips of the coleoptiles of wheat seedlings are removed and IAA in lanolin applied. Controls are conducted with plain lanolin, untreated cut coleoptiles and intact coleoptiles

14 Effect of indoleacetic acid on maize coleoptiles

Indoleacetic acid in lanolin is applied to one side of a maize coleoptile. A control with plain lanolin is included

15 The effect of light on shoots

Pea seedlings are grown for a week in light or darkness and their shoots compared

16 Respiratory activity in maize seedlings

Maize grains and seedlings are immersed in tetrazolium chloride solution for 30 minutes

 

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